Anti-KDM8 Antibody Picoband™

Product Name

Anti-KDM8 Antibody Picoband™

View all JMJD5 Antibodies

SKU/Catalog Number

A08840-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-KDM8 Antibody Picoband™ catalog # A18099. Tested in WB, ELISA applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-KDM8 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08840-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

E.coli-derived human KDM8 recombinant protein (Position: D4-D406). Human KDM8 shares 78.1% and 78.9% amino acid (aa) sequence identity with mouse and rat KDM8, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A08840-2 is reactive to Kdm8 in Human

Applications

A08840-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

55 kDa

Calculated molecular weight

47.145kDa

Background of JMJD5

Lysine demethylase 8 is a protein that in humans is encoded by the KDM8 gene. This gene likely encodes a histone lysine demethylase. Studies of a similar protein in mouse indicate a potential role for this protein as a tumor suppressor. Alternatively spliced transcript variants have been described.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of KDM8 using anti-KDM8 antibody (A08840-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KDM8 antigen affinity purified polyclonal antibody (Catalog # A08840-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KDM8 at approximately 55 kDa. The expected band size for KDM8 is at 47 kDa.

Gene/Protein Information For Kdm8 (Source: Uniprot.org, NCBI)

Alternative Names

EC 1.14.11.27; FLJ13798; JmjC domain-containing protein 5; jumonji domain containing 5; Jumonji domain-containing protein 5; KDM8; lysine-specific demethylase 8 Kdm8|3110005O21Rik, Jmjd5|lysine (K)-specific demethylase 8|bifunctional peptidase and arginyl-hydroxylase JMJD5|L-arginine (3R)-hydroxylase KDM8|arginyl C3-hydroxylase KDM8|jmjC domain-containing protein 5|jumonji C domain-containing protein 5|jumonji domain containing 5|jumonji domain-containing protein 5|lysine-specific demethylase 8

For more info on Kdm8, check out the Kdm8 Infographic

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In this infographic, you will see the following information for Kdm8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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